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. 2000 Nov 21;97(24):13027–13032. doi: 10.1073/pnas.97.24.13027

Figure 5.

Figure 5

The 27SA3 pre-rRNA accumulates during Rlp7p depletion. RNA was isolated from the pGAL1∷RLP7 strain after growth in galactose (lane 3) or 17 h (lane 4) and 20 h (lane 5) after switching to growth in glucose. RNA was isolated from an otherwise isogenic strain, YPH259, after growth in galactose (lane 2) or 24 h after the switch to growth in glucose (lane 1). Primer extension with the oligonucleotide A2/A3 that hybridizes to ITS2 was used to reveal processing sites A2, A3, B1L, and B1S. Primer extension with the oligonucleotide C1 that hybridizes to the 25S rRNA was used to map the 5′ end of the 25S rRNA. Products of the primer extension reactions were resolved on polyacrylamide gels next to a DNA sequencing ladder (not shown for 25S) and exposed to x-ray film.