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Preresonance Raman spectra (excited at 1024 nm, Bruker FRA 106/s) of DMPC/DMPA liposomes containing GR variants at room temperature. (A) Wild-type at pD 9 and pH 9 (0.1 M NaCl + 0.05 M KH2PO4 + 0.05 M CHES), and at pH 3 (0.1 M NaCl + 0.05 M KH2PO4 + 0.05 M sodium citrate); (B) E132Q at pH 5 (0.1 M NaCl + 0.05 M KH2PO4 + 0.05 M MES) and pH 9, and D121N at pH 9.
