Dependency maps of (a) EF–(0Ca-CaM), (b) EF–(2Ca-CaM), and (c) EF–(4Ca-CaM). The EF-CaM complex is schematically represented by its seven pseudodomains, with CaM (pseudodomains N-CaM and C-CaM) with dots and EF (pseudodomains CA, CB, SA, SC, Hel) with horizontal lines. The arrows represent the energetic influences: the larger the greater intensity or number of lost ranks in the comparison of free binding energies (light shading is 1, medium shading is 2, dark shading is 3, and solid is 4).