Table 6.

Reference binding free energies of the seven pseudodomains of EF–(2Ca-CaM)

	ΔΔEele	ΔΔEvdw	ΔΔEint	ΔΔGsolpolar	ΔΔGsolnonpolar	ΔΔG
CA	−552 ± 87	−314 ± 11	52 ± 4	800 ± 85	11 ± 3	−3 ± 22
CB	−407 ± 60	−82 ± 5	24 ± 3	497 ± 58	14 ± 2	44 ± 13
SA	−1410 ± 91	−241 ± 9	20 ± 3	1526 ± 84	17 ± 3	−89 ± 24
SC	−881 ± 45	−241 ± 9	19 ± 3	1049 ± 43	2 ± 2	−51 ± 20
Hel	−1911 ± 78	−161 ± 9	10 ± 2	2035 ± 75	21 ± 2	−6 ± 17
N-CaM	−1387 ± 86	−101 ± 11	10 ± 2	1445 ± 82	25 ± 2	−9 ± 18
C-CaM	−705 ± 87	−251 ± 12	10 ± 2	830 ± 77	16 ± 3	−101 ± 29

The different contributions to the total free energy of binding ΔΔG are given in kcal/mol as the Coulomb interaction energy (ΔΔE_ele), the van der Waals energy (ΔΔE_vdw), the internal energy (ΔΔE_int), the polar solvation energy (ΔΔG_sol^polar), and the nonpolar solvation energy (ΔΔG_sol^nonpolar).