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Effect of Met substitutions on the thermodynamics of peptide binding to CaM. ΔΔH (black bars) or TΔΔS (gray bars) values, in comparison to wt-CaM, are shown for Met-substituted CaM proteins binding to the smMLCKp or NtMKP1b peptides. For each protein binding to the smMLCKp peptide, values are shown for experiments performed at 25°C, 17.6°C, and 10.2°C (viewed left to right).
