Figure 2.

SANS analysis of VWF subjected to fluid shear. (A) Setup for shear experiments employing SANS. 0.6 nm neutrons are scattered by protein sample placed in a Couette cell. Area detectors placed at either 1.95 or 10.05 m detect scattered neutrons. (B) I(q) versus q (q = 4 πsin(θ/2)/λ) plot for experiment 1. Scattering intensity before shear is shown by empty circles and during shear (G = 3000/s) by solid circle. The applied shear profile is in the inset. Changes in scattering intensity are observed at both low-q (<0.08, large length scales) and high-q (>0.6, small length scales). q^∗ denotes the highest scattering vector value beyond which intensity increases upon shear application. (C) Normalization of scattering intensity (I(q)) measured in panel B by concentration (c) measured during the experiment (i.e., I/c versus q plot). Change in scattering intensity at high-q (q > 0.6/nm) suggest alterations in structural features at length scales <10 nm. Error bars in panel B are standard deviations. These error bars are very small and are thus not visible in the case of most data points.