Table 3.
Comparison of calculated KD values for binding of pY-peptides to Y317-Shc or pY317-Shc
| Shc | pY0992 | pY1086 | pY1114 | pY1148 | pY1173 |
|---|---|---|---|---|---|
| Y317 | 54.840 (10.521) | 58.744 (9.711) | 0.076 (0.017) | 0.192 (0.045) | 295.953 (118.055) |
| pY317 | 643.444 (150.634) | 245.675 (55.215) | 5.678 (1.727) | 0.853 (0.288) | 1705.140 (352.179) |
| p values | 0.001∗ | 0.002∗ | 0.002∗ | 0.009∗ | 0.001∗ |
| Binding domain | SH2 | PTB | PTB | PTB | SH2 |
KD values (in μM) were calculated as KD = exp(ΔG/RT), where ΔG represents the binding free energies reported in Tables 1 and 2. R = 1.987 (cal K−1 mol−1), and T = 300 K. Standard deviations are shown in parentheses.
∗
Statistical significance of differences in KD values between Y317-Shc and pY317-Shc was determined by Student's t test. Differences are considered statistically significant at p < 0.01.