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. 2009 Mar 18;96(6):2237–2244. doi: 10.1016/j.bpj.2008.12.3909

Figure 7.

Figure 7

Possible distributions of states for WT and R146 mutants. (A) Occupancy of the intermediate state as a function of assumed ATPase rates for the intermediate state (vx). At any given vx, the fractional population of the intermediate state is higher for the mutations than for the WT. The activities of the inactive and active states are assumed to be 0.1/s and 6/s, respectively. An ATPase rate less than 0.26/s would require a fraction larger than 1.0 for the intermediates state in the case of R146W, limiting vx to this minimum rate. B and C show the fraction of states in the intermediate and active states for different assumed fractions in the inactive state in the presence of saturating calcium. (B) Possible distributions for the minimum possible value of vx, 0.26/s, with “X” indicating the intermediate state and “A” representing the active state. (C) The same as B but with vx = 0.85/s. At this point, the lowest possible fraction of the inactive state for the R146G mutation in saturating calcium is 0.5.