Figure 6.
(a) Intermolecular Ie/Im for PPDPC (X =0.01) measured for MLVs composed of POPC and the indicated contents of C16-ceramide (■) or C16-C1P (○). (b) Fluorescence anisotropy (r) for DPH (X = 0.002) residing in binary MLVs composed of POPC and C16-ceramide (■) or C16-C1P (○). The total lipid concentration was 22.5 μM in 20 mM HEPES, 0.1 mM EDTA, and 150 mM NaCl (pH 7.4). The temperature was maintained at 25°C with a circulating waterbath.