Figure 5.

DLS measurements at 25°C of 30-nm extruded (A) DOBMP vesicles and (B) POPC vesicles as a function of time. For these experiments, lipids were hydrated only at neutral pH because lipid degradation (detected via TLC) occurs over the 5-week time period for DOBMP vesicles under acidic conditions. Results of SDS solubilization leakage assays of BMP 400-nm extruded vesicles (100 mM NaCl) loaded with (C) calcein, pH 7.4, and (D) ANTS/DPX, pH 4.2. Error bars represent the standard deviation from three different titration experiments. The dashed lines are guides for the reader. Note that the concentrations of the lipid solutions used in A and B were 200 μM and 50 μM, respectively, which accounts for the variation in concentration (x axis values), needed to solublize the vesicles.