Fig. 4.
Stable expression of full-length (A2B) and truncated version (A2B-316) of the A2B adenosine receptor in HEK 293. aSolid lines represent saturation isotherm for specific binding of [3H]-ZM241385 to membranes from stably transfected HEK 293 cells. Dashed lines represent non-specific binding. Data are means from duplicate determinations. b NECA-dependent cAMP accumulation. Data are results from six experiments presented as mean ± SEM. Asterisks indicate significant difference from full-length A2B receptors for each NECA concentration (*p < 0.05, **p < 0.01, unpaired two-tailed t test)