Fig. 3.

Ependymal cell response to BzATP is absent in P2X₇^−/− mice. a The genotype of C57Bl/6 (P2X₇^+/+) and P2X₇^−/− mice was confirmed by PCR [(+/+) 363 bp, (−/−) 280 bp]. CD1 wild-type mice also show a 363-bp product (data not shown). b, c Prolonged (60 s) applications of BzATP induce inward currents in P2X₇^+/+ (b) but not P2X₇^−/− (c) mice (bars = 100 pA, 20 s). d Summary histogram showing the average current density of BzATP-induced responses in P2X₇^+/+ mice (9.0 ± 1.1 pA/pF; n = 8) and lack of currents induced in P2X₇^−/− mice (0.01 ± 0.06 pA/pF; n = 16, *p < 0.05). e, f Ependymal P2X₇ immunoreactivity (green) is present in P2X₇^+/+ mice (e) but is absent in P2X₇^−/− mice (f). Also shown are GLAST (red) and doublecortin (blue; bar = 25 μm)