Table 1.

Primers used in RT-PCR of ependymal cells and animal genotyping

Target	Primer	Basic T(m), °C	Product size (bp)	Restriction enzyme (cleaved sizes in bp)
RT-PCR
P2X7	5′ TCTTCCGACTAGGGGACATCT 3′	54	119	BglII (82, 37)
	5′ ATGGGACCAGCTGTCTAGGTT 3′	54
Genotyping
P2X7+/+	5′ TGGACTTCTCCGACCTGTCT 3′	54	363	n.a.
	5′ TGGCATAGCACCTGTAAGCA 3′	52
P2X7−/−	5′ CTTGGGTGGAGAGGCTATTC 3′	54	280	n.a.
(neo insert)	5′ AGGTGAGATGACAGGAGATC 3′	52