Fig. 5.

Basal and cytokine-modulated expression of ART2.1 and ART2.2 in splenic T cells and BW5147 thymocytes. a T cells were isolated from BALB/c spleens by negative selection (immuno-depletion of B cells and myeloid cells) and were either assayed for ecto-ART activity immediately after isolation or after in vitro incubation for 12 or 24 h in the absence or presence of IFN-γ (100 U/ml). T cells were assayed for ecto-ART activity as described in Fig. 4c. b T cells were isolated from C57BL/6 spleens by negative selection and were assayed for ecto-ART activity immediately after isolation or after in vitro incubation for 15 or 24 h. T cells were assayed for ecto-ART activity as described in Fig. 4c. c BW5147 T cells were stimulated without or with IFN-β (100 U/ml) or IFN-γ (100 U/ml) for 24 h before extraction and RT-PCR analysis for ART2 (total), ART2.1, ART2.2, IRF-1, and GAPDH mRNA content. d BW5147 T cells were incubated without or with IFN-γ (100 U/ml) for 24 h (priming incubation) prior assay of ecto-ART activity as described in Fig. 4c. All results are representative of observations from two to three independent experiments