Table 1. SNPs investigated in this study together with the PCR primers and detection method.
Gene | Chrom. | SNP | NCBI A.N° | AA substitution | Base change | Detection method | PCR primers | AT | FS (bp) | DFS (bp) |
MTHFR | 1 | c.203G>A | rs2066472 | p.R68Q | A/G | PCR+TaqI dig | 5′CGA TTG GAA TCT GGT GAC AA3′ 5′ACC TTG CAT GAG TTT ACC TTG3′ | 56°C | 101 | 48+53 |
c.665C>T | rs1801133 | p.A222V | C/T | PCR+HinfI dig | 5′CCA AAG GCC ACC CCG AAG3′ 5′GAA AGA TCC CGG GGA CGA TG3′ | 56°C | 180 | 75+105 | ||
c.1286A>C | rs1801131 | p.E429A | A/C | PCR+MboII dig | 5′CTT TGG GGA GCT GAA GGA CTA CTA C3′ 5′CAC TTT GTG ACC ATT CCG GTT TG3′ | 56°C | 163 | 56+31+30+28+18 | ||
MTRR | 5 | c.66A>G | rs1801394 | p.I22M | A/G | PCR+NdeI dig | 5′GCA AAG GCC ATC GCA GAA GAC3′ 5′TGG TGG TAT TAG TGT CCT TTT3′ | 56°C | 179 | 22+157 |
c.524C>T | rs1532268 | p.S175L | C/T | PCR+TaqI dig | 5′CGT GGA TTG CTG GAC TCT G3′ 5′AGC AGC TCT GAC TTC ACA AGG3′ | 56°C | 133 | 101+32 | ||
CBS | 21 | c.919G>A | - | p.G307S | A/G | PCR+PvuII dig | 5′ATC ATT GGG GTG GAT CCCGA 3′ 5′ACC GTG GGG ATG AAG TCG CAG3′ | 60°C | 113 | 92+21 |
Chrom: Chromosome.
SNP: single nucleotide polymorphism.
NCBI AN: NCBI Accession Number.
AA: Amino Acid.
Dig: Digestion.
AT: Annealing Temperature.
FS: Fragment size.
DFS: Digestion Fragment size.