Table 1. SNPs investigated in this study together with the PCR primers and detection method.

Gene	Chrom.	SNP	NCBI A.N°	AA substitution	Base change	Detection method	PCR primers	AT	FS (bp)	DFS (bp)
MTHFR	1	c.203G>A	rs2066472	p.R68Q	A/G	PCR+TaqI dig	5′CGA TTG GAA TCT GGT GAC AA3′ 5′ACC TTG CAT GAG TTT ACC TTG3′	56°C	101	48+53
		c.665C>T	rs1801133	p.A222V	C/T	PCR+HinfI dig	5′CCA AAG GCC ACC CCG AAG3′ 5′GAA AGA TCC CGG GGA CGA TG3′	56°C	180	75+105
		c.1286A>C	rs1801131	p.E429A	A/C	PCR+MboII dig	5′CTT TGG GGA GCT GAA GGA CTA CTA C3′ 5′CAC TTT GTG ACC ATT CCG GTT TG3′	56°C	163	56+31+30+28+18
MTRR	5	c.66A>G	rs1801394	p.I22M	A/G	PCR+NdeI dig	5′GCA AAG GCC ATC GCA GAA GAC3′ 5′TGG TGG TAT TAG TGT CCT TTT3′	56°C	179	22+157
		c.524C>T	rs1532268	p.S175L	C/T	PCR+TaqI dig	5′CGT GGA TTG CTG GAC TCT G3′ 5′AGC AGC TCT GAC TTC ACA AGG3′	56°C	133	101+32
CBS	21	c.919G>A	-	p.G307S	A/G	PCR+PvuII dig	5′ATC ATT GGG GTG GAT CCCGA 3′ 5′ACC GTG GGG ATG AAG TCG CAG3′	60°C	113	92+21

Chrom: Chromosome.

SNP: single nucleotide polymorphism.

NCBI AN: NCBI Accession Number.

AA: Amino Acid.

Dig: Digestion.

AT: Annealing Temperature.

FS: Fragment size.

DFS: Digestion Fragment size.