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. 2009 Aug 6;4(8):e6537. doi: 10.1371/journal.pone.0006537

Figure 1. Representative flow cytometry analysis performed at day 0, 15, and 30 of culture.

Figure 1

A significant number of ASCs at day 0 are clearly positive for mesenchymal markers, including CD29 (89%), CD105 (12%), CD34 (33%), CD90 (52%), CD117 (10%), and for endothelial markers, including CD31 (9%), CD133 (4%), CD44 (5%), CD54 (9%), and Flk-1 (32%). At day 15 the CD34 and CD90 levels consistently increased (80%). After 30 days of culture, CD34 levels significantly decreased down to 8%, while the CD90 expression held steady at 80%. Later, cells were mainly positive for CD44 and CD54 antigens (87%). Control PE-conjugated and FITC-conjugated isotypes were negative and all cells were negative for CD45 antigens.