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. 2009 Aug 6;4(8):e6537. doi: 10.1371/journal.pone.0006537

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De Francesco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Image showing differentiated endothelial cells that, after 48 h in methylcellulose, acquire a different morphology comprising round cells with cytoplasmic granules and large flat cells with endothelial morphology (Original magnification 400×); (B) Image showing differentiated endothelial cells that, after day 7 in methylcellulose, formed an extensive intercellular tube networks (Original magnification 400×); Immunofluorescence images of differentiated endothelial cells showing positivity for several specific markers including (C) CD90 (Original magnification 100×); (D) VEGF (Original magnification 100×); (E) CD31 (Original magnification 100×); (F) Immunohistochemistry images of differentiated endothelial cells showing positivity for VEGF (Original magnification 100×); (G) At 140 hours, VEGF quantity was found to be of 5130 pg/ml with respect to the value of 336 pg/ml found at 12 hours; (H) At day 12, VEGF was 6331 pg/ml of medium with respect to 382 pg/ml found at day 1.