Figure 3. Incidence of gonadal apoptosis (TUNEL assay) in cortisol-treated (0.8 mg/g food) larvae between 4 and 6 wah.

Apoptosis occurrence is defined as the percentage of individuals showing each of the categories of apoptosis indicated in the left side of the top panels (red: abundant, yellow: few, grey: none; the right side shows DAPI nuclear staining for visualization of cells). Scale bars represent 10 µm; numbers within parenthesis indicate sample number. Cortisol-treated larvae showed markedly higher incidence of gonadal apoptosis compared to control larvae at 6 wah.