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. 2004 Jun 20;42(2):85–89. doi: 10.3347/kjp.2004.42.2.85

Fig. 2.

Fig. 2

PCR analysis of the SNU-H1 human isolate with subsequent passages in different animal hosts using 1.2% agarose gels stained with ethidium bromide. A. PCR using the C. parvum-specific primer. A 238 bp fragment was amplified in all samples. B. Genotype-specific PCR using the genotype 1- and 2-specific primers. In all samples, 312 bp and 411 bp fragments were amplified. Lane M, DNA size marker; lane 1, the human isolate SNU-H1; lane 2, 1st passage of the SNU-H1 in immunosuppressed C57BL/6 mice; lane 3, 2nd passage in a calf inoculated with the 1st passage.