Table 2.

NFAT cycling model parameters and initial conditions

Symbol	Units	Value	Source
Ccn	Dimensionless	50.0	(see Methods)
Kd,1	nM	1760	(see Supporting Material)
kf,1	nM−1.s−1	7.69 × 10−6	(Fitted value)
kr,1	s−1	1.93 × 10−2	(Fitted value)
kf,2	s−1	1.44 × 10−3	(Fitted value)
kf,3	s−1	3.62 × 10−4	(Fitted value)
kr,3	nM−1.s−1	4.71 × 10−5	(Fitted value)
kf,4	s−1	4.45 × 10−4	(Fitted value)
Km,N	nM	535	(see Supporting Material)
M	nM	6000	(21)
n	Dimensionless	2.92	(see Supporting Material)
Ntot	nM	1000	(21)
Steady-state values of the NFAT species depend on the protocol applied.
For the protocol of Shen et al. (11) and the dual-calcium protocol, use
NFATpc	nM	9.83 × 10−1	(Steady-state)
NFATpn	nM	2.76 × 10−1	(Steady-state)
NFATNc	nM	1.70 × 10−3	(Steady-state)
NFATNn	nM	5.09 × 10−1	(Steady-state)
For the protocol of Shen et al. (11) only
τN	s	7.01 × 10−2	(Fitted value)
tN, inhib	s	3600	(Protocol)
trainPeriod	s	50.0	(Protocol)
stimEnd	s	3600	(Protocol)
For the dual-calcium protocol only
period	s	> 0.017	(User-defined)
For the protocol of Tomida et al. (15), use
NFATpc	nM	9.96 × 10−1	(Steady-state)
NFATpn	nM	7.79 × 10−2	(Steady-state)
NFATNc	nM	4.81 × 10−4	(Steady-state)
NFATNn	nM	1.09 × 10−1	(Steady-state)
stimEnd	s	1800	(Protocol)