. 2009 Jun 16;28(8):1265–1272. doi: 10.1007/s00299-009-0726-y

Table 1.

Analysis of expression and inheritance of two endosperm-specific transgenes [uidA and sgfp(S65T)] after crossing between transgenic barley plants, GPBhGN-7 (T₈) and GPDhGFP-12 (T₄)

Crossed line	F₁ plant	Expression in F₂ seed
Crossed line	uidA/sgfp(S65T) PCR (±)	# of seeds examined	GUS (+/−)	GFP (+/−)
GPBhGN/DhGFP-2	+/+	96	71/25^†	77/19^†
GPBhGN/DhGFP-5	+/+	96	80/16^†	78/18^†
GPBhGN/DhGFP-6	+/+	96	70/26^†	70/26^†

^†Analyses of transgene segregation ratio of F₂ seed using χ²-test were not significantly different from 3:1 (at a = 0.05)