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Effect of pH on the gelatin-degrading activity of the 80 kDa protease in ESP from Toxoplasma gondii. The buffers (10 mM) used were MES for pH 5.5, PIPES for pH 6.5, HEPES for pH 7.5, Tris-HCl for pH 8.5, CHES for pH 9.5, and CAPS for pH 10.5.
