Amplified DNA profiles of Acanthamoeba spp. using a primer of 730. Y4, Acanthamoeba YM-4; A. cul, Acanthamoeba culbertsoni; A. hat, Acanthamoeba hatchetti; A. tri, Acanthamoeba triangularis; A. pol, Acanthamoeba polyphaga. L, DNA ladder marker (Hong et al., 1995).