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Experimental design
a)The chickens of group 1 were inoculated intragastrically with 1 × 106 oocysts after preservation (2-40 months) at intervals of 2-months, respectively.
b)The chickens of group 2 were infected with freshly harvested oocysts at intervals of 2-months, respectively.
c)Fecal collection from 10 chickens.
d)Five chickens were sacrificed.
