Fig. 3.

Examples of PCR amplification for the locus-specific typing of CsRn1. PCRs performed with locus-specific (see Table 3) and CsRn1 specific primers (5'-GAAACTTGAAGTGAGCAAC-3') by using the genomic DNAs of C. sinensis individually extracted. CsRn1-containing loci and their subset numbers in parentheses are presented. A. Agarose gel analysis of the PCR products. After being electrophoresed, the amplicons were visualized by ethidium bromide staining; B. Autoradiographs of the PCR products probed with the LTR sequence of CsRn1. Numbers on top of each lane represent individual worms of C. sinensis. M, 100-bp DNA ladder. C, positive control with each corresponding lambda clone as template. Template control reactions were performed to check the relative amounts and purities of each template DNA with primers for a cysteine protease (GenBank accession No. AF271091, 5'-GCTGGACTCCGACTACCCATATG-' and 5'-GGTTTAAACGATTGTGCATCGC-3').