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Experimental setup for analysis of elastic and inelastic light scattering from a single optically trapped spore. A near-infrared (NIR) laser beam at 785 nm is introduced in an inverted microscope (Nikon TE2000 DIC) equipped with an objective by a dichroic mirror to form a single-beam optical trap. A germinating spore in liquid is trapped in the focus of the laser beam about 10 µm above the coverslip.
