Table 2.
Values of Tlag, ΔTrelease, T1, and T2 for individual spores germinating in L-alanine or CaDPA. 10 spores of strains PS832, FB111, FB112, and FB113 were germinated with L-alanine and 5 spores of PS533 were germinated with L-alanine or CaDPA as described in Fig. 3–5, and values for Tlag, ΔTrelease, T1, and T2 were determined as described in Methods. Values in parentheses are the percentage of initial Ca-DPA remaining in spores at Tlag.
| Strain | Tlag – min | ΔTrelease – min | T1 - min | T2 - min |
|---|---|---|---|---|
| PS533 (wt) in L-alanine | 12.0 ± 4.5 (78.0) | 2.7 ± 0.6 | 10.7 ± 4.1 | 13.4 ± 4.8 |
| PS832 (wt) in L-alanine | 6.3 ± 1.7 (82.3) | 3.7 ± 0.8 | 4.6 ± 2.6 | 8.0 ± 2.3 |
| PS533 (wt) in CaDPA | 33.9 ± 7.0 (87.8) | 2.3 ± 0.2 | 12.7 ± 4.4 | 35.1 ± 7.4 |
| FB113 (cwlJ sleB) in L-alanine | 9.5 ± 3.2 (89.5) | 42.9 ± 13.3 | 21.0 ± 13.0 | Not observed |
| FB111 (cwlJ) in L-alanine | 33.5 ± 5.8 (78.9) | 40.7 ± 8.6 | 24.1 ± 2.4 | Not observed |
| FB112 (sleB) in L-alanine | 36.7 ± 9.8 (68.1) | 3.6 ± 2.1 | 34.4 ± 8.9 | 38.2 ± 9.9 |