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. 2009 Jan 2;3(1):012005. doi: 10.1063/1.3058587

Figure 5.

Figure 5

(a) A schematic 3D view of PCR chip composed of three layers. The upper layer is PDMS with a 13 mm×9 mm groove in the center which is loaded with 20 μl PCR mixture. The middle layer is a glass slide with a thickness of 140 μm. The lower layer is PDMS embedded with microheater, microthermal sensor and cooling channel. (b) Ultraviolet images of the PCR product for the chip (lane 1) and the conventional thermal cycler (lane 2). Lane 3 is a 1 kbp DNA ladder (Invitrogen), with an arrow indicating the 506-bp fragment of the 1 kbp DNA ladder, used as the reference.