Figure 1.
Electrophoresis of PCR products spanning the BRCA1 and hMLH1 promoters from bisulfite-treated DNA in breast cancer tissues. Each lane contains products generated from separate PCR reactions using primers specific for unmethylated (U) or methylated (M) DNA template. Fully methylated DNA from Active Motif and Blood from healthy individual used as a positive control (C) for methylated and unmethylated DNA (B). The lanes marked (H2O) indicates water only without the addition of DNA and (L) 100-bp ladder as molecular weight markers (Fermentas).