Table 1.
Summary of primer sequences, annealing temperature, product size and number of cycle used in MSP assay. M: methylated DNA, U: unmethylated DNA, F: forward, R: Reverse.
| Gene | Sequence (5′-3′) | Tm (°C) | Size (bp) | PCR cycles | |
|---|---|---|---|---|---|
| hMLH1 (U) | F | TTTTGATGTAGATGTTTTATTAGGGTTGT | 60 | 114 | 30 |
| R | ACCACCTCATCATAACTACCCACA | ||||
| hMLH1 (M) | F | ACGTAGACGTTTTATTAGGGTCGC | 58 | 110 | 30 |
| R | CCTCATCGTAACTACCCGCG | ||||
| BRCA1I (U) | F | GGTTAATTTAGAGTTTTGAGAGATG | 60 | 75 | 30 |
| R | TCAACAAACTCACACCACACAATCA | ||||
| BRCA1 (M) | F | GGTTAATTTAGAGTTTCGAGAGACG | 60 | 86 | 30 |
| R | TCAACGAACTCACGCCGCGCAATCG |