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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mech Ageing Dev. 2009 Jun 25;130(8):538–546. doi: 10.1016/j.mad.2009.06.006

Fig. 6. Compative uptake and killing of P. gingivalis in young vs. aged macrophages.

Fig. 6

(A) Mouse macrophages were incubated for 30 min with FITC-labeled P. gingivalis strains (MOI = 10:1). Association (i.e., representing both adherence and phagocytosis) or phagocytic indices were determined by flow cytometry, as outlined in Materials and Methods, using the following formula: % positive cells for FITC-P. gingivalis x MFI/100. (B–C) Mouse macrophages were infected with P. gingivalis (MOI = 10:1) and viable CFU of internalized bacteria were determined using an intracellular survival assay at 1.5h and 15h postinfection (B). After 15 h, macrophages were also assayed for production of NO2 (stable metabolite of NO) using a Griess reaction-based kit (C). Data are means (with SD) of triplicate determinations. Asterisks indicate statistically significant (p < 0.05) differences between “old” and “young” macrophages.