Figure 3.

TGN-accumulated Na pump is rapidly trafficked to the lateral membrane. (A) SNAP cells were subjected to BG-Block, incubated at 37°C for 30 min to begin synthesis of new Na pump, and placed at 19°C for 2 h to accumulate newly synthesized protein in the TGN. TMR-STAR is depicted in red, and Golgi markers are shown in green. (B) SNAP cells were treated as in A and either fixed immediately (19°C) or warmed to 31°C for the indicated times. Samples were labeled with TMR-STAR (red) and with anti-HA (blue). Bars, 5 µm.