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. 2009 Jul 27;186(2):193–200. doi: 10.1083/jcb.200905016

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© 2009 Hofmann et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Localization of EYFP-actin in live cells. (A) Distribution of EYFP-NLS-actin-wt and EYFP-NLS-actin-K68/284R. Confocal images of live cells taken 20 h after transfection (left). The distribution of the two proteins was quantified in 100 transfected cells per experiment (error bars indicate mean ± SEM, n = 3). (B) Time course of cellular localization of EYFP-actin-wt and EYFP-NLS-actin-K68/284R after leptomycin B treatment (2 ng/ml).