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. 2009 Jul 27;186(2):243–254. doi: 10.1083/jcb.200902119

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© 2009 Landeira et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Knockdown of TbSCC1 results in the in situ transcriptional activation of inactive telomeric VSG ESs. (A) Histogram showing the relative expression of mRNA measured by qRT-PCR of VSG genes located in different VSG ESs (ES) or different promoter-lacking VSG BC genes upon 4 d of induction of TbSCC1 RNAi. An increase in the expression of VSG genes located in previously inactive VSG ESs is detected, whereas VSG-BCs remain at a similar level when compared with the uninduced control. Expression values are plotted in relative expression units, calculated relative to myosin expression, and multiplied by 1,000 for better visualization on a logarithmic scale. (B) Histogram showing the fold change in gene expression levels (TbSCC1 RNAi induced/TbSCC1 RNAi uninduced) shown in A. (A and B) Mean ± SD of three different measures is shown.