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. 2009 Jul 27;186(2):229–241. doi: 10.1083/jcb.200902101

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© 2009 Zhou et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — zyg-12(ct350ts) disrupts the regular arrangement of germline nuclei. (A and B) Schematic lateral (A) and transverse (B) view of germline nuclear position in the C. elegans gonad. (C and D) DAPI-stained germline nuclei are regularly arrayed in the distal gonad arm of adult wild-type animals (C) but disarrayed in zyg-12(ct350ts) gonads (D). (E and F) Immunofluorescent images with ZYG-12 in green, α-tubulin in red, and nuclei (DAPI) in blue. Oocytes contain a single nucleus in the proximal gonad of wild-type animals (E) but have multiple nuclei in zyg-12(ct350ts) gonads (F). All animals were shifted to 25°C during L1.