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. 2009 Jul 27;186(2):173–182. doi: 10.1083/jcb.200903100

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© 2009 Amano et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Kinetochore localization of the CENP-S–CENP-X complex occurs downstream of the CENP-H complex but is distinct from the CENP-O complex. (A) CENP-X localization in CENP-S–deficient cells (CENP-S OFF) and CENP-S localization in CENP-X–deficient cells (CENP-X OFF). WT, wild type. (B) CENP-S localization in CENP-K– or CENP-T–deficient cells (CENP-K OFF or CENP-T OFF, respectively). (C) Immunofluorescence analysis in wild-type DT40 and CENP-50– and CENP-X–deficient cells with the indicated antibodies (green). DNA was counterstained with DAPI (blue). (D) Imaging of human HeLa cells stably expressing GFP-tagged CENP-H after siRNA-based knockdown for control or CENP-X. Bars, 10 µm.