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. 2009 Aug;134(2):137–150. doi: 10.1085/jgp.200910232

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© 2009 Estevez et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — A comparison of time courses of ROL production derived from microspectrophotometry versus microfluorometry. Data from Figs. 3 (bottom) and 4 have been replotted on the same graph for comparison. Data obtained by microspectrophotometry (MSP) are shown in black: n = 11 cells (native), 9 cells (9-DM), and 11 cells (11-cis). Fluorescence data are shown in gray: n = 6 cells (native), 6 cells (9-DM), and 5 cells (11-cis). Concentration of ROL in microspectrophotometry data are expressed as a fraction of bleached cone pigment. Fluorescence data are scaled for best visual match to microspectrophotometry. Error bars show ± SEM.