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. 2009 Aug;134(2):151–164. doi: 10.1085/jgp.200910240

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© 2009 Martínez-François et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Gating effects of mutations at Y352 and E363 are not additive. (A) Macroscopic currents of the Y352A/E363Q double mutant in 2 mM cGMP; each trace is the average of nine individual traces. (B) Corresponding G-V curve (mean ± SEM; n = 5). (C) Residual conductance (mean ± SEM; n = 4–5) at very negative voltages of the channels containing a single E363Q (squares) or Y352A (circles) mutation, or a Y352A/ E363Q double mutation (triangles).