Figure 3.

Effect of proteasome inhibitors on PR activity. In A, a pulse–chase similar to Fig. 1 was conducted in HeLa cells transfected with the PR mutant pD25A. Relevant parts of the fluorograms depicting cell and virus fractions are shown (Right), and calculation of release kinetics is depicted (Left). (B) Recombinant Pr55 and PR were incubated in the presence or absence of inhibitors at the concentrations indicated. Cleavage reactions were analyzed by Western blot by using HIV-1 CA-specific antiserum.