Figure 4. Shh signaling disrupted in staggerer.

Q-PCR demonstrates reduced expression of Shh, Gli1, and Ptch RNA in staggerer cerebellum. Shh and Gli1 are significantly altered (p ≤0.05) at E15.5, while Ptch is not. All three genes are significantly different at both E17.5 and P0 (p ≤0.05).