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. Author manuscript; available in PMC: 2009 Jul 29.
Published in final edited form as: Neuron. 2003 Dec 18;40(6):1119–1131. doi: 10.1016/s0896-6273(03)00769-4

Figure 5. Exogenous SHH stimulates staggerer granule cell precursor proliferation.

Figure 5

(A) rSHH is sufficient to induce proliferation in sg granule precursors. 300 μm sections from a P4 staggerer were cultured in the absence (left) or presence (right) of rSHH. After 2 days, dividing cells were labeled by BrdU incorporation for 24 hours, then fixed and visualized with a FITC-conjugated antibody under epifluorescence.

(B) Diminished proliferation of neonatal sg granule precursors is overcome by rSHH. 300 μm sections of wild-type and staggerer cerebellum at P0 were cultured immediately in media containing BrdU in the presence or absence of exogenous SHH. After 3 days in culture, sections were fixed, incubated with FITC-conjugated anti-BrdU antibody, and visualized by epifluorescence.

(C) Fluorescence density was measured across each section in using MetaMorph software. Average values from multiple sections ± s.e.m. are shown. * p ≤0.05; ** p ≤0.01.