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. Author manuscript; available in PMC: 2009 Jul 29.
Published in final edited form as: Neuron. 2003 Dec 18;40(6):1119–1131. doi: 10.1016/s0896-6273(03)00769-4

Figure 6. RORα regulates Purkinje cell genes involved in calcium signaling.

Figure 6

(A) Line plots include normalized array data from Pcp4, Slc1A6, Itpr1, and Cals1. In situ hybridization at postnatal day 2 (E15.5 for Pcp4) indicates the relevant cell population and qualitatively confirms decreased expression. Quantitative real-time PCR at P0 demonstrates reduced expression for Cals1, Slc1A6 and Itpr1. Difference by genotype was significant for all three genes (p≤0.01).

(B) SCA1 target genes that are relevant to calcium signaling are decreased in staggerer as well. Q-PCR showed 5-phosphatase levels decreased at P0, when Serca2/Atp2a2 is not reliably detected. Both genes are decreased in adult. Difference by genotype is significant for 5-phosphatase in both P0 (p ≤0.05) and adult (p ≤0.025) as is Serca2 (p≤0.03).