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. Author manuscript; available in PMC: 2009 Jul 29.
Published in final edited form as: Neuron. 2003 Dec 18;40(6):1119–1131. doi: 10.1016/s0896-6273(03)00769-4

Figure 7. RORα and coactivator binding and activation of target genes.

Figure 7

ChIP experiments on freshly dissected cerebella demonstrate in vivo binding of RORα to Pcp4, Pcp2, Slc1A6, Shh (at two sites), and Itpr1 promoters, but not to Baf53a and a second site in the Pcp2 promoter. RORα recruits specific sets of coactivators to each target promoter, including Tip60, β-catenin, and p300. None of the examined coactivators were bound to the Itpr1 promoter. Location of the PCR product relative to the known or presumed start of transcription is indicated for each gene; arrowhead indicates putative RORα binding site. (For the Shh distal site, a space in the image indicates different gels).

(B) Co-immunoprecipitation assays were performed on freshly dissected cerebella from P0 mice. β-catenin was pulled down with an RORα antibody but not a control IgG from the same host species. β-catenin was detected with two separate antibodies.

(C) Microinjection of blocking antibodies against β-catenin, SRC-1, and Tip60, but not CBP, p/CIP, and p/CAF blocked activation of the Pcp2/LacZ reporter in the presence of a CMV-RORα expression construct.