Fig. 2. CrebH is expressed in the adult liver and gastrointestinal tract.
A) RT-PCR analyses of CrebH and Hnf4α was performed on mRNA extracts from the adrenal gland (Adr), brain (Brn), colon (Col), duodenum (Ddn), heart (Hrt) ileum (Ile), kidney (Kid), liver (Liv), lung (Lng), ovary (Ovr), stomach (Sto), testis (Tes) and uterus (Utr). B-G) The distribution of CrebH mRNA in the liver (B, E), small intestine (C, F), and stomach (D, G), was identified (white silver grains) using radioactive in situ hybridization analysis. CrebH mRNA was present in the hepatocytes (h, arrow) of the liver, the epithelial cells of the villi (v,arrow), but not the crypts (c, white arrows; a yellow dashed line demarcates the villi/crypt border) of the small intestine, and in the surface lining cells (sl) of the stomach. H&E-stained bright field images (B, C, D) and corresponding dark field images (E, F, G) are presented.