Table 2. Steady-State Kinetic Parameters for One-Base Incorporation by Dpo4.
| template base | dNTP | kcat (s−1) | Km,dNTP (×103 μM) | kcat/KM (×103 μM−1 s−1) | fold reduction from dCTP:dG |
|---|---|---|---|---|---|
| Substrate 1 with dC 5′ of G/M1dG | |||||
| dG | dCTP | 160 ± 5 | 3.2 ± 0.6 | 49 | − |
| dATP | 20 ± 2 | 260 ± 90 | 0.075 | 650 | |
| dTTP | 35 ± 1 | 200 ± 40 | 0.18 | 270 | |
| dGTP | 16 ± 2 | 70 ± 40 | 0.22 | 220 | |
| M1dG | dCTP | 9.3 ± 1.7 | 120 ± 90 | 0.080 | 610 |
| dATP | 24 ± 1 | 130 ± 20 | 0.19 | 260 | |
| dTTP | 3.8 ± 0.3 | 110 ± 30 | 0.034 | 1400 | |
| dGTP | 1.1 ± 0.1 | 30 ± 5 | 0.035 | 1400 | |
| Substrate 2 with dT 5′ of G/M1Dg | |||||
| dG | dCTP | 890 ± 30 | 4.3 ± 0.5 | 210 | − |
| dATP | 22 ± 1 | 120 ± 20 | 0.18 | 1.200 | |
| dTTP | 58 ± 2 | 230 ± 40 | 0.25 | 840 | |
| dGTP | 19 ± 1 | 400 ± 120 | 0.048 | 4400 | |
| M1dG | dCTP | 29 ± 2 | 400 ± 80 | 0.073 | 2900 |
| dATP | 15 ± 1 | 30 ± 5 | 0.49 | 430 | |
| dTTP | 15 ± 1 | 130 ± 30 | 0.12 | 1800 | |
| dGTP | 16 ± 2 | 180 ± 70 | 0.089 | 2400 | |