Table 3. Steady-State Kinetic Parameters for Next-Base Extension past M1dG by Dpo4.
| primer pair | dNTP | kcat (s−1) | Km,dNTP (×103 μM) | kcat/KM (×103 μM−1 s−1) | fold reduction from dC:dG extension |
|---|---|---|---|---|---|
| Substrates Containing dC 5′ of G/M1dG | |||||
| dC·dG | dGTP | 420 ± 10 | 5.0 ± 0.7 | 84 | − |
| dA·dG | dGTP | 24 ± 1 | 52 ± 5 | 0.47 | 180 |
| dC·M1dG | dGTP | 6.6 ± 0.3 | 2.2 ± 0.5 | 2.9 | 29 |
| dA·M1dG | dGTP | 8.3 ± 0.5 | 13 ± 4 | 0.64 | 130 |
| Substrates Containing dT 5′ of G/M1dG | |||||
| dC·dG | dATP | 1100 ± 30 | 14 ± 1 | 78 | − |
| dA·dG | dATP | 2.1 ± 0.1 | 64 ± 18 | 0.033 | 2400 |
| dC·M1dG | dATP | 28 ± 1.0 | 5.6 ± 1.0 | 5.0 | 16 |
| dA·M1dG | dATP | 1.4 ± 0.1 | 56 ± 13 | 0.025 | 3100 |
| dA·M1dG | dTTP | 2.6 ± 0.3 | 13 ± 7 | 0.2 | 390 |