Table 1. Fucα(1−2)Gal Glycoproteins Identified from the Mammalian Olfactory Bulb.
| protein | function | accession no. | MW (Da) | peptide no. | sequence coverage (%) |
|---|---|---|---|---|---|
| Cell Adhesion Molecules | |||||
| NCAM1 | cell adhesion, axonal outgrowth, and fasciculation | IPI00831465.1 | 93474 | 21 | 33.5 |
| IgSF3 | cell adhesion and neuronal fasciculation | IPI00420589.2 | 134605 | 21 | 18.6 |
| contactin-1 precursor | cell adhesion and neuronal fasciculation | IPI00123058.1 | 113372 | 10 | 10.2 |
| NCAM L1 | cell adhesion and neuronal fasciculation | IPI00406778.3, IPI00785371.1, IPI00831568.1 | 140413 | 9 | 10.7 |
| OCAM (NCAM2) | cell adhesion and neuronal fasciculation | IPI00127556.1, IPI00322617.1 | 93187 | 7 | 9.0 |
| NRCAM | cell adhesion and neuronal fasciculation | IPI00120564.5, IPI00338880.3, IPI00395042.1, IPI00403536.1 | 122736 | 7 | 6.3 |
| kirrel2 | cell adhesion and neuronal fasciculation | IPI00471420.1, IPI00623939.2 | 72754 | 5 | 10.3 |
| Ion Channels and Solute Transporters/ Carrier Proteins | |||||
| slc12a2 | chloride transport | IPI00135324.2 | 130654 | 15 | 14.9 |
| cacna2d1 | calcium channel | IPI00230013.3, IPI00319970.1, IPI00407868.1, IPI00410982.1, IPI00626793.3 | 122505 | 13 | 13.0 |
| aralar1 | Asp, Glu exchanger | IPI00308162.3 | 74553 | 12 | 16.7 |
| slc3a2 | cationic amino acid transporter | IPI00114641.2 | 58805 | 8 | 16.7 |
| slc7a5 | cationic amino acid transporter | IPI00331577.3 | 55856 | 5 | 8.6 |
| VDAC3 | voltage-dependent anion channel | IPI00122548.3, IPI00762642.1 | 30867 | 5 | 16.2 |
| ATP-Binding Proteins/ ATP Synthase | |||||
| Na+/K+-ATPase α1 | maintenance of the electrochemical gradient | IPI00311682.5 | 112967 | 34 | 35.4 |
| ATP synthase α subunit | ATP synthesis | IPI00130280.1 | 59736 | 11 | 23.7 |
| Na+/K+-ATPase β1 | maintenance of the electrochemical gradient | IPI00121550.2 | 35771 | 6 | 25.2 |
| vacuolar ATP synthase catalytic subunit A | ATP synthesis | IPI00407692.3 | 68309 | 6 | 13.1 |
| ATP synthase γ chain | ATP synthesis | IPI00313475.1, IPI00750074.1, IPI00751391.1, IPI00775853.1, IPI00776084.1, IPI00776275.1 | 30239 | 6 | 24.1 |
| ATP synthase B chain | ATP synthesis | IPI00341282.2 | 28931 | 6 | 19.9 |
| Na+/K+-ATPase α3 | maintenance of the electrochemical gradient | IPI00122048.2 | 111676 | 6 | 18.3 |
| ATP-binding cassette, subfamily C, member 9 | efflux of endogenous and xenobiotic molecules | IPI00111686.5 | 148816 | 5 | 3.4 |
| Synaptic Vesicle Proteins | |||||
| munc18 (syntaxin-binding protein 1) | synaptic vesicle cycling | IPI00415402.3 | 67553 | 13 | 23.6 |
| synaptotagmin-1 | synaptic vesicle cycling | IPI00129618.1, IPI00750142.1 | 47400 | 9 | 24.0 |
| NSF vesicle-fusing ATPase | synaptic vesicle cycling | IPI00656325.2 | 82598 | 8 | 11.7 |
| Mitochondrial Proteins | |||||
| prohibitin-2 | protein folding | IPI00321718.4 | 33279 | 7 | 24.4 |
| prohibitin | protein folding | IPI00133440.1 | 29802 | 7 | 29.4 |
| ubiquinol−cytochrome c reductase complex core protein 2 | enzyme | IPI00119138.1 | 48218 | 6 | 19.0 |
| mitochondrial inner membrane protein isoform 1 | unknown | IPI00228150.1, IPI00381412.1, IPI00554845.1 | 83883 | 6 | 11.1 |
| Other | |||||
| plexin B2 | cell signaling receptor | IPI00405742.6, IPI00666301.1, IPI00752396.1 | 206212 | 8 | 4.2 |
| LINGO1 | cell signaling coreceptor | IPI00134200.1, IPI00750473.1 | 69085 | 6 | 10.3 |
| MARCKS | cell signaling | IPI00229534.5 | 29643 | 5 | 17.2 |
| insulin-like growth factor 1 receptor | cell signaling receptor | IPI00120225.1 | 155772 | 5 | 3.1 |
a
Proteins from C57BL/6 mice were captured by UEAI affinity chromatography in three separate experiments.
b
The number of sequence unique, full tryptic peptide identifications returned from a reversed database searching strategy. See for procedures and analysis criteria.