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. 2009 Aug;23(8):2702–2709. doi: 10.1096/fj.08-118919

TABLE 1.

Physical parameters of various BRET systems developed to date using mutant Renilla luciferases

BRET system	Donor				Acceptor
BRET system	Reporter	Substrate	Em_max	Filter	Reporter	Ex_max	Em_max	Filter
GFP2-Rluc8	Rluc8	Clz400	400 nm	360–450 nm	GFP2	400 nm	511 nm	500–570 nm
Rluc8-YFP	Rluc8	Clz/Enduren	480 nm	20 nm spectral	YFP	514 nm	527 nm	20 nm spectral
mOrange-Rluc8	Rluc8	Clz/Enduren	480 nm	400–520 nm	mOrange	548 nm	564 nm	550–620 nm
mOrange-Rluc8.6	Rluc8.6	Clz/Enduren	535 nm	20 nm spectral	mOrange	548 nm	564 nm	20 nm spectral

Em_max, maximum emission; Ex_max, maxiumum excitation.