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. 2009 Aug;23(8):2595–2604. doi: 10.1096/fj.08-122424

Figure 2.

Figure 2.

Effect of isoflurane anesthesia on tau kinases and phosphatases in 4.4-mo-old homozygous JNPL3 female mice. Extracts from brain stem of control mice (Ctl; lane 1, n=6) and mice sampled at the end of 4 h of anesthesia (Anes; lane 2, n=5), or 1 wk after (A+1w; lane 3, n=6) were separated by SDS-PAGE and identified with antibodies indicated in boxes. A) Inhibited GSK-3β (Ser9). B) Total GSK-3β. C) Activated JNK (pThr183 and pTyr185). D) Total JNK. E) Activated MAPK (pThr202 and pTyr204). F) Total MAPK. G) cdk5. H) p35. I) Activated CaMKII (pThr286). J) Total CaMKII. K) Activated Akt (pSer473). L) total Akt. M) PP1 catalytic subunit. N) PP2B catalytic subunit. O) PP2A catalytic subunit. P) PP2A activity assay. Scatterplots represent quantification of the immunoblot bands displayed above them (except P, which displays PP2A activity). Levels of phosphokinases (A, C, E, I, K) were normalized to total kinase levels. Graphs show results expressed as percentage of control (100%). Numbers in graphs indicate percentage of the tick line with which they are aligned. Data represented are means ± sd (1 representative value displayed; each lane represents an individual mouse). *P < 0.05, **P < 0.01 vs. Ctl; ANOVA with Neumans-Keuls post hoc test.