Figure 3.

Successful mRNA and protein knockdown of STIM1, Orai, and TRPC using siRNA. A–F) Western blot analysis probing for decrease protein levels of STIM1 (A), Orai (B, C), and TRPC isoforms (D–F) after RNA silencing. Actin was used as a control, and densitometry was performed using ImageJ software (for Orai3 blots, densitometry is shown for siOrai3-1). Extended versions of Western blots are shown in Supplemental Fig. 2. G) qPCR assessing effect of specific siRNA on Orai2 mRNA levels. Western blotting data are representative of 3 independent experiments; qPCR data are representative of 6 independent experiments. Wt, wild type.